Evaluation of commercially available third-generation anti-hepatitis C virus enzyme-linked immunosorbent assay in patients on haemodialysis.
Indian J Med Microbiol
;
2007 Apr; 25(2): 140-2
Artículo
en Inglés
| IMSEAR
| ID: sea-53806
ABSTRACT
Restricted antibody reactivity to hepatitis C virus (HCV) synthetic peptides has been observed in HCV-infected patients on haemodialysis (HD). The aim of this study was to evaluate third-generation anti-HCV enzyme-linked immunosorbent assay (ELISA) test systems containing either synthetic peptide HCV antigens or recombinant HCV antigens or a combination of synthetic and recombinant antigens in screening of 69 chronic renal failure patients on HD for HCV infection. Seven patients were detected to have antibodies to HCV by the 'recombinant HCV antigens'-containing kits, of which the recombinant immunoblot assay for HCV confirmed four cases. The recombinant kits had a sensitivity of 100% and a specificity of 66%. However, the ELISA kits with only synthetic HCV antigens failed to detect antibodies in any of the cases (zero sensitivity). Hence a recombinant protein containing ELISA test system is ideal for screening of HCV infection in patients on hemodialysis.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Péptidos
/
Proteínas Recombinantes
/
Humanos
/
Ensayo de Inmunoadsorción Enzimática
/
Sensibilidad y Especificidad
/
Diálisis Renal
/
Hepatitis C
/
Anticuerpos contra la Hepatitis C
/
Insuficiencia Renal
/
Antígenos Virales
Tipo de estudio:
Estudio diagnóstico
/
Estudios de evaluación
Idioma:
Inglés
Revista:
Indian J Med Microbiol
Asunto de la revista:
Microbiology
Año:
2007
Tipo del documento:
Artículo
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