DNA fingerprinting elicited evolutionary trend of oral Candida tropicalis isolates from diverse geographic locales.
Indian J Med Microbiol
;
2006 Jul; 24(3): 186-94
Artículo
en Inglés
| IMSEAR
| ID: sea-53998
ABSTRACT
PURPOSE:
To study molecular profiles of oral Candida tropicalis isolates from five different geographic locales to determine the molecular diversity, clonality and evolutionary trends of this opportunistic pathogen.METHODS:
A total of 36 strains from five countries (China, Canada, Scotland, Japan and Tanzania) were genotyped by PCR fingerprinting with 11 separate primers. Of these, primers RSG9, RSG8, T3B and RSD12 generated complex fingerprinting patterns.RESULTS:
Three significantly dissimilar profiles were derived from the primer T3B and particularly focused on tDNA suggested the prevalence of genetic subtypes within the species. Comparison of tDNA and rDNA (RSD12) fingerprints of C. tropicalis suggested that rDNA is much more heterogeneous than the relatively distinct tDNA. Further analysis of similarity coefficient (SAB) of gel profiles derived from computer-generated dendrograms indicated some degree of similarity in isolates from five-disparate geographic locales as well as the presence of unique isotypes in each region.CONCLUSIONS:
This study demonstrates the evolutionary divergence of distinct genetic subgroups within Candida tropicalis .
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Humanos
/
Candidiasis Bucal
/
ADN de Hongos
/
ARN Ribosómico
/
ARN de Transferencia
/
Dermatoglifia del ADN
/
Infecciones Oportunistas Relacionadas con el SIDA
/
Técnica del ADN Polimorfo Amplificado Aleatorio
/
Candida tropicalis
Idioma:
Inglés
Revista:
Indian J Med Microbiol
Asunto de la revista:
Microbiology
Año:
2006
Tipo del documento:
Artículo
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