Application of ATP assay for in vitro drug screening testing against human derived M. leprae.
Indian J Lepr
;
1989 Jul; 61(3): 333-44
Artículo
en Inglés
| IMSEAR
| ID: sea-54214
ABSTRACT
In this study, the ATP content of M. leprae exposed to various antimicrobial agents has been measured to evaluate its usefulness in drug sensitivity screening. Purified M. leprae suspensions from human biopsies have been incubated at 30 degrees C in a modified Dubos medium in the presence of different concentrations of various drugs viz., Rifampicin, Ethionamide, Ethambutol, Cycloserine, Dapsone, Clofazimine, Erythromycin and Tetracycline. ATP levels were estimated at 0, 7 days, 14 days of incubation by the procedures modified and standardised at this laboratory. ATP decay was accelerated by ethionamide, rifampicin, clofazimine, dapsone, erythromycin and to a lesser extent by cycloserine, whereas ethambutol and tetracycline did not have any significant effect. The rate of decay depended on the concentrations of these drugs. ATP assay promises to be a useful system for in vitro drug sensitivity screening against M. leprae isolated from patients.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Fotometría
/
Humanos
/
Pruebas de Sensibilidad Microbiana
/
Adenosina Trifosfato
/
Relación Dosis-Respuesta a Droga
/
Leprostáticos
/
Lepra
/
Animales
/
Mycobacterium leprae
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Inglés
Revista:
Indian J Lepr
Asunto de la revista:
Tropical Medicine
Año:
1989
Tipo del documento:
Artículo
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