Morphology of M. leprae (?) in VS3E medium--a preliminary communication.

ABSTRACT

In a previous attempted culture of M. leprae in VS2M medium non-acid fast organisms were seen initially and acid fast organisms appeared later. A drop of a sonicated suspension from a subculture of this was inoculated in VS3E medium. The inoculum consisted mostly of acid fast granules. The culture yielded pure growth of acid fast organisms. Morphology typical of M. leprae could be seen only after 60th day of culture.