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Comparison of biochemical characterisation of ICRC bacilli with M. leprae: effect of substrate alteration in the medium.
Indian J Lepr ; 1984 Apr-Jun; 56(2): 212-8
Artículo en Inglés | IMSEAR | ID: sea-55576
ABSTRACT
ICRC-bacilli strain C-44 when grown in Dubos medium of its equivalent, express M. avium taxonomic biochemical characters. Assuming that difference in characters of M. leprae and ICRC bacilli, could be due to 'in vivo' and 'in vitro' milieu, we altered the substrates in the medium. The bacilli grow well in the new medium containing selenium, ferric nitrate, magnesium chloride and deleting Tween 80. The ICRC strain C-44 grown in new medium expressed characters 9/10 similarity with M. leprae. The 10 day tween hydrolysis reaction in weak but positive. It is probable that 'M. leprae culture isolate', may have acquired 'in vitro' growth potential by recombination with M. avium, an ubiquitous mycobacterium. The M. leprae culture isolate thus may express some characters of both M. leprae and M. avium.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Medios de Cultivo / Mycobacterium / Mycobacterium avium / Mycobacterium leprae Idioma: Inglés Revista: Indian J Lepr Asunto de la revista: Tropical Medicine Año: 1984 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Medios de Cultivo / Mycobacterium / Mycobacterium avium / Mycobacterium leprae Idioma: Inglés Revista: Indian J Lepr Asunto de la revista: Tropical Medicine Año: 1984 Tipo del documento: Artículo