Mini transposon vector mediated foreign gene expression in Mesorhizobium huakuii subsp. rengei.
Indian J Exp Biol
;
2004 Oct; 42(10): 1028-31
Artículo
en Inglés
| IMSEAR
| ID: sea-55740
ABSTRACT
Among the transposable elements, mini-Tn5 transposon vector has proven to be of greater utility for insertion mutagenesis of variety of Gram negative bacteria. The mini-Tn5 vector containing promoter less egfp gene and gentamycin resistant gene was used for the present study. The transposon vector was introduced to M. huakuii from E. coli S17 by conjugation. The conjugants were screened for stable expression of egfp both in free-living and in nodules of Astragalus sinicus. The result showed that the conjugant #3 showed stable expression of green fluorescent both in free-living and bacteroid stage. The visualization of sym plasmid of wild strain and conjugants showed that conjugant #3 had a fragmentation of large sized plasmid into two but without affecting the nodulating ability. These results clearly indicated that mini-Tn5 vectors (Transposon vectors) the best alternate tools for plasmid vectors for integration of foreign genes in chromosomal DNA or symbiotic plasmid and expression, both in free-living and bacteroid stage of Rhizobium.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Proteínas Recombinantes
/
Elementos Transponibles de ADN
/
Expresión Génica
/
Conjugación Genética
/
Alphaproteobacteria
/
Planta del Astrágalo
/
Proteínas Fluorescentes Verdes
/
Escherichia coli
/
Vectores Genéticos
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
2004
Tipo del documento:
Artículo
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