Isolation and characterization of a green tissue-specific promoter from pigeonpea [Cajanus cajan (L.) Millsp.].
Indian J Exp Biol
;
2005 Apr; 43(4): 369-72
Artículo
en Inglés
| IMSEAR
| ID: sea-56216
ABSTRACT
Expression of rbcS genes encoding small subunit of rubisco, most abundant protein in green tissue, is regulated by at least three parameters--tissue type, light conditions and stage of development. One of the green tissue-specific promoters of rbcS gene family was isolated from pigeonpea by PCR. Expression of uidA gene encoding beta-glucuronidase in the transgenic tobacco plants under the control of pigeonpea rbcS promoter, clearly showed that this promoter was as strong as pea rbcS3A promoter characterized earlier. Study of the sequence similarity with pea rbcS3A promoter, especially the region (boxes I and III) that is required for rbcS3A expression, showed more than 50% divergence. In contrast, pigeonpea promoter sequence isolated in the present study was more similar to that of spinach and rice rbcS promoters.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Regiones Promotoras Genéticas
/
Plantas Modificadas Genéticamente
/
Genes de Plantas
/
Hojas de la Planta
/
ADN de Plantas
/
Cajanus
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
2005
Tipo del documento:
Artículo
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