Curing of symbiotic plasmid of Mesorhizobium huakuii subsp. rengei isolated from Astragalus sinicus.
Indian J Exp Biol
;
2003 Aug; 41(8): 912-4
Artículo
en Inglés
| IMSEAR
| ID: sea-56474
ABSTRACT
Astragalus sinicus (Chinese Milk vetch), a green manure leguminous plant, harbors Mesorhizobium huakuii subsp. rengei strain B3 in the root nodules. The visualization of symbiotic plasmid of strain B3 showed the presence of one sym plasmid of about 425 kbp. Curing of sym plasmid by temperature and acrydine orange was studied. Growing rhizobial cells at high temperature (37 degrees C) or treating the cells with acrydine orange at 50 mg/l eliminated sym plasmid of M. huakuii strain B3, which was confirmed by sym plasmid visualization and plant infection test of cured strains.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Plásmidos
/
Rhizobium
/
Simbiosis
/
Raíces de Plantas
/
Planta del Astrágalo
/
Fijación del Nitrógeno
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
2003
Tipo del documento:
Artículo
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