Effect of gamma-irradiation of Escherichia coli on cyclic AMP binding activity.

ABSTRACT

Cyclic AMP binding in the extract of E. coli was taken as a measure of cAMP receptor protein (CRP) present in the cells. The gamma-irradiation of cells caused a dose-dependent inhibition of cAMP binding activity indicating that CRP gene is affected by gamma-irradiation. The binding activity in cells irradiated with 240 Gy gamma-ray dose remained unaltered by post-irradiation incubation. This supports previous finding that the enhanced inhibition of the L-arabinose isomerase synthesizing capacity, following incubation of gamma-irradiated cells at higher doses, must be due to a different cause than the catabolite repression.