Detection of bluetongue virus genome segment 6 sequences by RT-PCR.
Indian J Exp Biol
;
1998 Oct; 36(10): 1034-7
Artículo
en Inglés
| IMSEAR
| ID: sea-56968
ABSTRACT
Reverse transcription-PCR (RT-PCR) technique was adopted to amplify a 101 basepair nucleotide sequence of bluetongue virus (BTV) genome segment 6. The specificity of the amplicon was determined by its approximate size in 3% agarose gel electrophoresis, digestion with restriction enzyme MspI, dot-blot hybridization and cycle sequencing. The technique was found to be suitable for detection of bluetongue virus in infected cell culture and clinical samples.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Células Cultivadas
/
Genoma Viral
/
Virus de la Lengua Azul
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
1998
Tipo del documento:
Artículo
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