Role of PGrs and inhibitors in induction and control of somatic embryogenesis in Themeda quadrivalvis.
Indian J Exp Biol
;
2009 Mar; 47(3): 198-203
Artículo
en Inglés
| IMSEAR
| ID: sea-58332
ABSTRACT
Somatic embryogenesis could be achieved in Themeda quadrivalvis (Linn.) O. Ktze -fodder grass species on MS medium supplemented with 2,4-D. Incorporation of putrescine in the medium stimulated embryogenesis, however its lower concentration stimulated production of non-regenerative callus. Other polyamines such as spermine and spermidine could not evoke similar response. Ascorbic acid used as antioxidant could not prevent browning in embryogenic cultures, however it stimulated embryogenesis. Inhibition of auxin polar transport by use of TIBA and HFCA reduced the embryogenic response significantly and produced distorted or abnormal embryos. Antiethylene substances such as AgNO3 and CoCl2 added in the medium adversely affected the process of embryogenesis and counteracting the stimulatory role of ethylene.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Reguladores del Crecimiento de las Plantas
/
Ácido Ascórbico
/
Semillas
/
Poliaminas Biogénicas
/
Germinación
/
Ácido 2,4-Diclorofenoxiacético
/
Relación Dosis-Respuesta a Droga
/
Ácidos Indolacéticos
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
2009
Tipo del documento:
Artículo
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