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Effect of hematoporphyrin derivative and light on sulfhydryl groups in brain tumour cells.
Indian J Exp Biol ; 1995 Oct; 33(10): 721-4
Artículo en Inglés | IMSEAR | ID: sea-58493
ABSTRACT
Effect of hematoporphyrin derivative (HpD) and light on sulfhydryl (SH) groups in brain tumor cells was studied. Sulfhydryl groups were measured by 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) and a fluorescent probe 3,4-maleimidylphenyl-4-methyl-7-diethylaminocoumarin (CPM). Incubation of cells with HpD in dark resulted in the loss of DTNB as well as CPM reactive SH groups. After 2 hr of incubation DTNB reactive SH groups showed a negligible change while a continuous decrease was observed in CPM reactive SH groups. Cells treated with HpD showed a further degradation of SH groups upon light irradiation. A comparison of cytotoxicity and SH groups under identical conditions showed that blockage of SH groups by HpD binding is not leathal to the cells where as photoinduced cell death was observed on photodegradation of SH groups.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Fotoquimioterapia / Compuestos de Sulfhidrilo / Neoplasias Encefálicas / Humanos / Células Tumorales Cultivadas / Derivado de la Hematoporfirina Idioma: Inglés Revista: Indian J Exp Biol Año: 1995 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Fotoquimioterapia / Compuestos de Sulfhidrilo / Neoplasias Encefálicas / Humanos / Células Tumorales Cultivadas / Derivado de la Hematoporfirina Idioma: Inglés Revista: Indian J Exp Biol Año: 1995 Tipo del documento: Artículo