Partial characterization of serum immunoglobulins of Oreochromis mossambicus.
Indian J Exp Biol
;
2000 Jun; 38(6): 549-53
Artículo
en Inglés
| IMSEAR
| ID: sea-58497
ABSTRACT
Serum immunoglobulins of O. mossambicus were purified using chromatography methods--CM affinity gel blue chromatography followed by two step purification involving a combination of ion-exchange and gel filtration chromatography. Studies revealed that O. mossambicus produces only one class of high molecular weight macroglobulin as determined by molecular sieving by Sepharose CL 6-B. Immunoelectrophoresis of purified O. mossambicus serum against rabbit anti O. mossambicus serum gave only a single precipitin line. Further analysis of the immunoglobulin by SDS-PAGE showed that the IgM macroglobulin weighs about 900,000 Da, composed of mu-like heavy chain weighing about 90 kDa each and light chains weighing about 30 kDa each.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Precipitinas
/
Conejos
/
Electroforesis de las Proteínas Sanguíneas
/
Inmunoglobulina M
/
Inmunoglobulinas
/
Albúmina Sérica Bovina
/
Bovinos
/
Pruebas de Hemaglutinación
/
Inmunoelectroforesis
/
Cromatografía en Gel
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
2000
Tipo del documento:
Artículo
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