Comparison of two colorimetric assays to determine viral infectivity in micro culture virus titration.
Indian J Exp Biol
;
1999 Dec; 37(12): 1223-6
Artículo
en Inglés
| IMSEAR
| ID: sea-58690
ABSTRACT
Efficacy of two colorimetric assays, viz. MTT (3-4,5-dimethylthiazol-2-(yl-2,5-diphenyl tetrazolium bromide) and neutral red (NR) assays, performed by integrating them to micro culture virus titration (MCVT), was compared with the conventional MCVT method in terms of percentages of infectivity and 50% infectivity end points by employing Polio virus type-3 and Dengue virus type 4 as the candidate viruses. The results suggested that MTT assay has an edge over NR assay as well as conventional MCVT method. For the first time, NR assay has been successfully employed for the determination of virus infectivity titre.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Sales de Tetrazolio
/
Tiazoles
/
Virología
/
Células Vero
/
Humanos
/
Línea Celular
/
Chlorocebus aethiops
/
Colorimetría
/
Efecto Citopatogénico Viral
/
Poliovirus
Tipo de estudio:
Estudio diagnóstico
/
Estudios de evaluación
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
1999
Tipo del documento:
Artículo
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