Genetic transformation and hairy root induction in Holostemma ada-kodien K. Schum--a vulnerable medicinal plant.
Indian J Exp Biol
;
2001 Dec; 39(12): 1263-7
Artículo
en Inglés
| IMSEAR
| ID: sea-59112
ABSTRACT
Hairy roots were induced from shoot buds and seedling hypocotyls of Holostemma by infection with agropine type Agrobacterium rhizogenes strains. Type of explant, Agrobacterium rhizogenes strain used for infection, co-culture time and photoperiod influenced the transformation frequencies. Hairy roots were induced from seedling hypocotyls and shoot bud explants upon infection with agropine type Agrobacterium rhizogenes strains. The hairy roots were thin, whitish in colour and showed negatively geotropic growth. The transformed nature of hairy roots was confirmed by opine analysis.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Plantas Medicinales
/
Rhizobium
/
Transformación Genética
/
Raíces de Plantas
/
Apocynaceae
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
2001
Tipo del documento:
Artículo
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