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Somatic embryogenesis in Vigna radiata (L.) Wilczek.
Indian J Exp Biol ; 2000 Dec; 38(12): 1241-4
Artículo en Inglés | IMSEAR | ID: sea-59677
ABSTRACT
Somatic embryogenesis was achieved from immature cotyledon derived callus of mungbean, V.radiata (L.) Wilczek in MS liquid medium. Embryogenic callus was induced on MS medium with NAA (5 mg/L). Differentiation of somatic embryos was observed when embryogenic callus was transferred to MS liquid medium containing 2,4-D (1.5 mg/L) and L-proline (50 mg/L). The torpedo shaped embryos were transferred to MS liquid medium with BAP and ABA (1 mg/L each) for maturation and germination. Fifty per cent of torpedo shaped embryos were converted into tiny plants (8-9 plants out of 17) after one week of culture. The germinated embryos were isolated and transferred to MS half strength basal (solid) medium for further development.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Plantas Medicinales / Medios de Cultivo / Agricultura / Ácido 2,4-Diclorofenoxiacético / India / Fabaceae / Ácidos Naftalenoacéticos País/Región como asunto: Asia Idioma: Inglés Revista: Indian J Exp Biol Año: 2000 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Plantas Medicinales / Medios de Cultivo / Agricultura / Ácido 2,4-Diclorofenoxiacético / India / Fabaceae / Ácidos Naftalenoacéticos País/Región como asunto: Asia Idioma: Inglés Revista: Indian J Exp Biol Año: 2000 Tipo del documento: Artículo