In vitro capacitation of goat spermatozoa.
Indian J Exp Biol
;
1989 Nov; 27(11): 921-4
Artículo
en Inglés
| IMSEAR
| ID: sea-59788
ABSTRACT
Goat epididymal and ejaculated spermatozoa were incubated in Krebs-Ringer bicarbonate buffer containing pyruvate and lactate as energy source. A 3 hr incubation for epididymal and 4 hr for ejaculated spermatozoa was required for the capacitation and acrosome reaction to take place. Calcium is an essential requirement which was needed for motility maintenance/activation and for the initiation of acrosome reaction. A 2-fold increase in cAMP content was measured over 3 hr period of incubation of goat epididymal spermatozoa which was not seen when calcium ions were either omitted or chelated with EGTA. There is thus a definite involvement of Ca2+ ions and cAMP in capacitation and acrosome reaction of goat spermatozoa.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Capacitación Espermática
/
Motilidad Espermática
/
Espermatozoides
/
Acrosoma
/
Masculino
/
Cabras
/
Calcio
/
AMP Cíclico
/
Animales
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
1989
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS