Comparative evaluation of sensitivity of RNA-polyacrylamide gel electrophoresis and dot immunobinding assay for detection of bluetongue virus in cell culture.
Indian J Exp Biol
;
1999 Feb; 37(2): 157-60
Artículo
en Inglés
| IMSEAR
| ID: sea-60105
ABSTRACT
Bluetongue virus serotype 1 (Avikanagar isolate) was grown in BHK-21 cell line and titrated. The titre of the virus in BHK-21 cell line was 10(6) TCID50/ml. RNA-polyacrylamide gel electrophoresis (RNA-PAGE) and dot immunobinding assay (DIA) were performed on 10-fold serial dilutions of the sonicated cell culture material. The results indicated that the minimum limit of detection of the virus by RNA-PAGE and DIA was 10(5) TCID50/ml.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
ARN Viral
/
Immunoblotting
/
Línea Celular
/
Sensibilidad y Especificidad
/
Cricetinae
/
Virus de la Lengua Azul
/
Electroforesis en Gel de Poliacrilamida
/
Animales
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
1999
Tipo del documento:
Artículo
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