Detergent-mediated destaining of coomassie brilliant blue-stained SDS polyacrylamide gels.
Indian J Exp Biol
;
2001 Jan; 39(1): 95-7
Artículo
en Inglés
| IMSEAR
| ID: sea-60278
ABSTRACT
A simple and one-step detergent-mediated destaining procedure for SDS Polyacrylamide gels for proteins is described. Suspension (5%, w/v) of a commercially available household detergent, Vim Ultra, has been found to be very efficient in destaining polyacrylamide gels without interfering with the resolution of proteins. As compared to the routinely used solvent (methanol-acetic acid-water)-mediated destaining procedure, the present method is economical and user-friendly.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Colorantes de Rosanilina
/
Detergentes
/
Electroforesis en Gel de Poliacrilamida
/
Indicadores y Reactivos
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
2001
Tipo del documento:
Artículo
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