Immunological identification of two female-specific proteins from the plasma of Indian freshwater murrel, Channa punctatus (Bloch).
Indian J Exp Biol
;
2002 Mar; 40(3): 288-95
Artículo
en Inglés
| IMSEAR
| ID: sea-60418
ABSTRACT
Existence of a non-phosphorylated female-specific protein (FS II), in addition to phosphorylated vitellogenin (FS I), in the plasma of murrel by exogenous administration of estradiol-17beta is reported. Polyspecific rabbit antibodies were raised against estrogen-inducible murrel plasma proteins. This antiserum was absorbed with normal male serum in order to obtain female-specific antiserum (FSAS). Radial immunodiffusion studies suggested that both the proteins (FS I and FS II) were present in the plasma of E2-treated and normal vitellogenic females and in the ovarian homogenate from gravid females, but absent in normal male plasma. Autoradiographic experiments demonstrated that phosphorus moiety was attached with FS I only. Further, immunoelectrophoretic analysis and peptide maps supported the observation that FS I and FS II were discrete, unrelated female-specific proteins.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Perciformes
/
Femenino
/
Masculino
/
Proteínas Sanguíneas
/
Inmunoquímica
/
Vitelogeninas
/
Caracteres Sexuales
/
Animales
/
Especificidad de Anticuerpos
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
2002
Tipo del documento:
Artículo
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