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Survival of Shigella dysenteriae type 1 on fomites.
J Health Popul Nutr ; 2001 Sep; 19(3): 177-82
Artículo en Inglés | IMSEAR | ID: sea-605
ABSTRACT
Studies have shown that various objects, such as utensils, toys, and clothes, can serve as vehicles for transmission of Shigella spp. Shigellae can become viable but non-culturable (VBNC) when exposed to various environmental conditions as shown in earlier studies. The present study was carried out to detect VBNC Shigella dysenteriae type 1 on various fomites by direct viable counting, polymerase chain reaction (PCR), and fluorescent antibody methods. S. dysenteriae type 1 was inoculated onto cloth, wood, plastic, aluminum, and glass objects. Results showed that 1.5-4.0 hours after inoculation, S. dysenteriae type 1 became non-culturable, and after five days, non-culturable but viable S. dysenteriae type 1 could be detected by both PCR and fluorescent antibody techniques. Fomites can be considered an important potential route of transmission of VBNC S. dysenteriae type 1 and a significant factor in the epidemiology of shigellosis.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Shigella dysenteriae / Bangladesh / Humanos / Reservorios de Enfermedades / Recuento de Colonia Microbiana / Reacción en Cadena de la Polimerasa / Técnica del Anticuerpo Fluorescente / Disentería Bacilar País/Región como asunto: Asia Idioma: Inglés Revista: J Health Popul Nutr Asunto de la revista: Gastroenterology / Nutritional Sciences / Public Health Año: 2001 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Shigella dysenteriae / Bangladesh / Humanos / Reservorios de Enfermedades / Recuento de Colonia Microbiana / Reacción en Cadena de la Polimerasa / Técnica del Anticuerpo Fluorescente / Disentería Bacilar País/Región como asunto: Asia Idioma: Inglés Revista: J Health Popul Nutr Asunto de la revista: Gastroenterology / Nutritional Sciences / Public Health Año: 2001 Tipo del documento: Artículo