In vitro mass multiplication of Ophiorrhiza mungo Linn.
Indian J Exp Biol
;
2004 Jun; 42(6): 639-42
Artículo
en Inglés
| IMSEAR
| ID: sea-60740
ABSTRACT
A protocol for in vitro mass multiplication of plants through seedling (shoot) cultures was established for Ophiorrhiza mungo. Maximum number of adventitious shoots per shoot culture (10.4 +/- 1.72) was initiated on MS solid medium supplemented with BAP (2.22 microM) after 3 weeks. Shoots were further multiplied (12.8 +/- 2.8) through subculture of intact shoots and reculture of nodal segments of aseptic shoots (6.5 +/- 0.94) in MS solid medium containing BAP (0.89 microM). Shoot elongation (1.27 +/- 0.12 cm) was achieved in the medium containing GA3 (1.44 microM) in two weeks. Rooting was favoured in basal agar medium supplemented with IBA (12.3 microM) plus NAA (1.07 microM). The plants were successfully established (100%) in the pots containing sand and top soil (11) mixture in a period of two weeks.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Reguladores del Crecimiento de las Plantas
/
Factores de Tiempo
/
División Celular
/
Dióxido de Silicio
/
Brotes de la Planta
/
Raíces de Plantas
/
Fenómenos Fisiológicos de las Plantas
/
Rubiaceae
/
Medios de Cultivo
/
Agar
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
2004
Tipo del documento:
Artículo
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