Cost effectiveness of colony lysis and colony PCR methods for screening of recombinant Escherichia coli colonies--a comparative study.
Indian J Exp Biol
;
2008 Oct; 46(10): 731-5
Artículo
en Inglés
| IMSEAR
| ID: sea-60779
ABSTRACT
Economizing the research protocols by using low cost technologies is the need of laboratories of developing world. Screening of recombinant E. coli colonies is the crucial step in gene cloning and expression studies. In the present study, the cost effectiveness of colony lysis method and colony PCR method in the screening of recombinant E. coli colonies was compared. The colony lysis method was 20 two times more cost effective and less time consuming and can be used to screen the recombinant E. coli colonies in large scale instead of colony PCR method.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Recombinación Genética
/
Reacción en Cadena de la Polimerasa
/
Técnicas Bacteriológicas
/
Técnicas Genéticas
/
Análisis Costo-Beneficio
/
Escherichia coli
Tipo de estudio:
Estudio diagnóstico
/
Evaluación Económica en Salud
/
Estudio de tamizaje
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
2008
Tipo del documento:
Artículo
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