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Somatic embryogenesis and plant regeneration in Gloriosa L.
Indian J Exp Biol ; 2001 Sep; 39(9): 943-6
Artículo en Inglés | IMSEAR | ID: sea-60860
ABSTRACT
Friable callus was initiated from shoot apices of Gloriosa superba L. on basal MS medium supplemented with 2, 4-D (4mg L(-1)) + Kn(5 mg L(-1)) + CH(10 mg L(-1)) + CW(20%). Subculture of callus on the same medium after 4-5 weeks showed induction of large number of somatic embryos, which was confirmed with histological studies. Development of embryoids in plantlet took place when the embryogenic callus was transferred to basal MS medium supplemented with BAP (5 mg L(-1)), CH(50 mg L(-1)) +CW(20%). Roots were developed by subculturing them on to the medium containing Kn or BAP (5 mg L(-1)) and IBA (4 mg L(-1)). Plantlets were successfully transferred to pots containing mixture of soil, sand and farmyard manure (211).
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Hidrolisados de Proteína / Regeneración / Semillas / Adenina / Caseínas / División Celular / Brotes de la Planta / Medios de Cultivo / Cinetina Idioma: Inglés Revista: Indian J Exp Biol Año: 2001 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Hidrolisados de Proteína / Regeneración / Semillas / Adenina / Caseínas / División Celular / Brotes de la Planta / Medios de Cultivo / Cinetina Idioma: Inglés Revista: Indian J Exp Biol Año: 2001 Tipo del documento: Artículo