Monoclonal antibodies against bovine growth hormone.
Indian J Exp Biol
;
1999 Jul; 37(7): 650-4
Artículo
en Inglés
| IMSEAR
| ID: sea-61113
ABSTRACT
A number of mouse x mouse hybridomas producing monoclonal antibodies (MAbs) against bovine growth hormone (bGH) were prepared by fusion of spleen cells from bGH-primed mice (Balb/c) with non-secretory mouse myeloma cells (PAIOP3) and characterized. MAbs obtained from three fusion experiments belonged to IgM, IgG1 and IgG2b class/subclass of antibodies. Cross-reaction studies indicated that generated antibodies were against three different epitopes of bGH. VIA6E8 (IgG1) and VIIB2E11C9 (IgM) did not cross-react with ovine prolactin (oPRL), ovine leutinizing hormone (oLH) and porcine follicle stimulating hormone. Antibody VIB3C9E8 (IgM) exhibited cross-reaction with oPRL and oLH. Antibody VIC1F9 (IgG2b) cross reacted with oPRL. All MAbs were against conformational epitopes of bGH.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Bovinos
/
Hormona del Crecimiento
/
Células Tumorales Cultivadas
/
Reacciones Cruzadas
/
Animales
/
Ratones
/
Ratones Endogámicos BALB C
/
Anticuerpos Monoclonales
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
1999
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS