Rapid sexing of bovine preimplantation embryos using polymerase chain reaction: production of calves with predetermined sex under field conditions.
Indian J Exp Biol
;
1998 Dec; 36(12): 1201-8
Artículo
en Inglés
| IMSEAR
| ID: sea-61502
ABSTRACT
Two to four blastomere size biopsies were obtained from each 6-day-old embryo of zebu and crossbred cattle for sex determination. The sex of the embryos was determined with a set of bovine Y-chromosome specific primer pairs by using polymerase chain reaction. Thirty two biopsied embryos after their sex was determined, when transferred fresh to synchronized recipients, resulted in 56.2% pregnancy rate. Sixteen healthy calves were born at full term, while 2 heifers aborted at mid-term from fresh embryo transfer. Simultaneously, 44 biopsied embryos which were kept frozen, were thawed at a later date and transferred to the previously synchronized recipients, thereby leading to 24 pregnancies (54.5%). Twenty-three healthy calves were born at full term, while 1 heifer aborted at mid-term from frozen-thawed embryo transfer. The pregnancy rates from both fresh and frozen-thawed biopsied embryos were comparable with that of controls (P > 0.05). Except for a single misidentification of a male calf as a female by our PCR assay (2.6%), the phenotypic sex of all the live born calves as well as the aborted fetuses was correctly matched with the PCR detection.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Análisis para Determinación del Sexo
/
Cromosoma Y
/
Femenino
/
Masculino
/
Embarazo
/
Bovinos
/
Secuencia de Bases
/
Reacción en Cadena de la Polimerasa
/
Cartilla de ADN
/
Desarrollo Embrionario
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
1998
Tipo del documento:
Artículo
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