Comparative diagnostic potentiality of ELISA and dot-ELISA in prepatent diagnosis of experimental Fasciola gigantica infection in cattle.
Indian J Exp Biol
;
2005 Jun; 43(6): 536-41
Artículo
en Inglés
| IMSEAR
| ID: sea-61577
ABSTRACT
A glycoprotein (27 kDa) was isolated from crude somatic antigen of Fasciola gigantica by two steps affinity chromatography and was used in early detection of experimental fasciolosis in cattle by indirect ELISA and in dot-ELISA formats. Although, anti-27 kDa antibodies could be detected after 3 weeks post infection (WPI) by dot - ELISA which was one week later than indirect ELISA. The test, dot-ELISA, was more convenient in field application. By the test (dot-ELISA) the infection could be equally detected in animals infected with 100, 200 and 300 metacercariae of F. gigantica with high sensitivity. Further, the antigen (27 kDa) was not found to react with goat sera infected with Paramphistomum epiclitum, which are giving strong reaction to homologous immature and mature fluke antigens of P. epiclitum.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Conejos
/
Sefarosa
/
Factores de Tiempo
/
Infecciones por Trematodos
/
Inmunoglobulina G
/
Bovinos
/
Ensayo de Inmunoadsorción Enzimática
/
Cabras
/
Anticuerpos Antihelmínticos
/
Glicoproteínas
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
2005
Tipo del documento:
Artículo
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