Your browser doesn't support javascript.
loading
High frequency in vitro propagation of Phyllanthus amarus Schum. & Thom. by shoot tip culture.
Indian J Exp Biol ; 2001 Nov; 39(11): 1184-7
Artículo en Inglés | IMSEAR | ID: sea-61915
ABSTRACT
With the aim of micropropagation of Phyllanthus amarus, an important medicinal herb, shoot tips were cultured in Murashige and Skoog's medium supplemented with kinetin/ BAP singly or in combination with IAA. Growth regulators at lower range (0.1-1.0 mg L(-1)) stimulated direct regeneration of shoots. Kinetin was superior to BAP and kinetin-IAA combination was more suitable than kinetin alone. About 15 shoots were yielded per explant after 30 days of culture in the medium containing kinetin and IAA both at 0.1mg L(-1). The cluster of proliferated shoots elongated and rooted simultaneously under the same treatment following another subculture, thus shortening the total time schedule of micropropagation. Shoot tips of regenerated shoots were continuously used to regenerate new shoots with periodic transfer to fresh medium resulting in a steady supply of normal, healthy plants without any deviation in the production rate during a continuous one year culture. Micropropagated plants were successfully established in soil with high survivality (80%).
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Plantas Medicinales / Botánica / Brotes de la Planta / Medios de Cultivo / Phyllanthus Idioma: Inglés Revista: Indian J Exp Biol Año: 2001 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Plantas Medicinales / Botánica / Brotes de la Planta / Medios de Cultivo / Phyllanthus Idioma: Inglés Revista: Indian J Exp Biol Año: 2001 Tipo del documento: Artículo