Verotoxic Escherichia coli (STEC) from beef and its products.
Indian J Exp Biol
;
2007 Feb; 45(2): 207-11
Artículo
en Inglés
| IMSEAR
| ID: sea-62194
ABSTRACT
In the present investigation, out of 27 (24.10%) strains of Escherichia coli isolated from 112 beef samples comprising raw meat (45), kabab (36) and kofta (31), 9 (33.33%) belonging to 7 different serotypes were verotoxic as tested by vero cell cytotoxic assay. Serotype O145 was the predominant STEC in raw meat. Interestingly, one STEC-O157 strain was also detected. All the STEC strains were positive for Stx genes by polymerase chain reaction showing stx2 (77.78%) to be most predominant followed by stx1 (22.22%). Phenotypic enterohaemolysin production on washed sheep blood agar supplemented with CaCl2 revealed 6 (66.67%) STEC strains to be positive. Presence of STEC in cooked beef products, viz., kabab and kofta appeared to be a matter of concern and potential threat to public health.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Células Vero
/
Bovinos
/
Serotipificación
/
Chlorocebus aethiops
/
Reacción en Cadena de la Polimerasa
/
Toxina Shiga
/
Proteínas de Escherichia coli
/
Escherichia coli
/
Proteínas Hemolisinas
/
Animales
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
2007
Tipo del documento:
Artículo
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