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Isolation and purification of an extracellular protease from a new strain of Bacillus subtilis, viz.NCIM 2711.
Indian J Exp Biol ; 2003 Jun; 41(6): 614-9
Article en En | IMSEAR | ID: sea-62421
A new extracellular protease having a prospective application in the food industry was isolated from Bacillus sUbtilis NCIM 2711 by (NH4)2SO4 precipitation from the cell broth. It was purified using DEAE-Cellulose and CM-Sephadex C-50 ion-exchange chromatography. With casein as a substrate, the proteolytic activity of the purified protease was found to be optimal at pH 7.0 and temperature 55 degrees C with Km 1.06 mg/ml. The enzyme was stable over a pH range 6.5-8.0 at 30 degrees C for 1 hr in presence of CaCl2 x 2H2O. At 55 degrees C, the enzyme retained 60% activity up to 15 min in presence of CaCl2 x 2H2O. EDTA and o-phenanthroline (OP) completely inhibited the enzyme activity while DFP, PMSF and iodoacetamide were ineffective. The enzyme was completely inhibited by Hg2+ and partially by Cd2+, Cu2+, Ni2+, Pb2+ and Fe2+. The OP inhibited enzyme could be reactivated by Zn2+ and Co2+ up to 75% and 69% respectively. It is a neutral metalloprotease showing a single band of 43 kDa on SDS-PAGE.
Asunto(s)
Texto completo: 1 Índice: IMSEAR Asunto principal: Endopeptidasas / Bacillus subtilis / Cromatografía por Intercambio Iónico / Electroforesis en Gel de Poliacrilamida Idioma: En Revista: Indian J Exp Biol Año: 2003 Tipo del documento: Article
Texto completo: 1 Índice: IMSEAR Asunto principal: Endopeptidasas / Bacillus subtilis / Cromatografía por Intercambio Iónico / Electroforesis en Gel de Poliacrilamida Idioma: En Revista: Indian J Exp Biol Año: 2003 Tipo del documento: Article