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An enzyme-linked immunosorbent assay (ELISA) to measure growth hormone level in serum and milk of buffaloes (Bubalus bubalis).
Indian J Exp Biol ; 2007 Jul; 45(7): 594-8
Artículo en Inglés | IMSEAR | ID: sea-62445
ABSTRACT
An indirect Sandwich ELISA to measure growth hormone level in serum and milk of buffaloes was developed. The assay was based on purified anti rbST IgG raised in rabbits and chicken and rabbit anti chicken IgG horseradish peroxidase. The assay was validated in terms of sensitivity, specificity, precision and recovery. Parallelism was demonstrated between the standard curve and serially diluted serum, milk and pituitary derived growth hormone. Sensitivity of the assay was 0.1 ng/ml. Recovery of exogenous bovine somatotropin from serum and milk ranged from 90 to 102% and 96 to 108% respectively. The intra and inter assay variations to measure growth hormone in serum and milk were 3.36 to 8.81% and 6.01 to 12.31% respectively. Statistical analysis for parallelism and cross-reactivity of rbST with serum of other species confirmed the reproducibility of the assay.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Conejos / Calibración / Inmunoglobulina G / Búfalos / Bovinos / Ensayo de Inmunoadsorción Enzimática / Hormona del Crecimiento / Pollos / Reproducibilidad de los Resultados / Modelos Estadísticos Tipo de estudio: Estudio diagnóstico / Estudio pronóstico / Factores de riesgo Idioma: Inglés Revista: Indian J Exp Biol Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Conejos / Calibración / Inmunoglobulina G / Búfalos / Bovinos / Ensayo de Inmunoadsorción Enzimática / Hormona del Crecimiento / Pollos / Reproducibilidad de los Resultados / Modelos Estadísticos Tipo de estudio: Estudio diagnóstico / Estudio pronóstico / Factores de riesgo Idioma: Inglés Revista: Indian J Exp Biol Año: 2007 Tipo del documento: Artículo