In vitro regeneration of Hypericum patulum Thunb.--a medicinal plant.
Indian J Exp Biol
;
2001 Sep; 39(9): 947-9
Artículo
en Inglés
| IMSEAR
| ID: sea-62624
ABSTRACT
A protocol was developed for high frequency plant regeneration in H. patulum by shoot-tip culture. H. patulum plants were collected from a wild source growing at high altitude in the eastern Himalayas. Multiple buds were initiated from shoot-tips cultured on Murashige and Skoog's medium supplemented with BAP, kinetin. Addition of thiamin HCI, Ca-pantothenate and biotin enhanced multiple shoot formation. Upon transfer to phytohormone free liquid medium following a brief exposure to auxin, root formation occurred from the micro shoots . Rooted plants were hardened and transferred to soil. Regeneration potentiality was found to be constant throughout the year in long term cultures.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Ácido Pantoténico
/
Reguladores del Crecimiento de las Plantas
/
Plantas Medicinales
/
Purinas
/
Regeneración
/
Tiamina
/
Biotina
/
Adenina
/
Brotes de la Planta
/
Raíces de Plantas
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
2001
Tipo del documento:
Artículo
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