Site specific integration of FLP recombinase in BHK-21 cell line.
Indian J Exp Biol
;
2003 Jun; 41(6): 570-3
Artículo
en Inglés
| IMSEAR
| ID: sea-62740
ABSTRACT
A binary system for gene activation and site specific integration based on conditional recombination of transfected sequences mediated by FLP recombinase from yeast was implemented in mammalian cells. In several cell lines, FLP rapidly and precisely recombined copies of its specific target sequences to activate an otherwise silent beta-galactosidase reporter gene. Clones of marked cells were generated by excisional recombination within a chromosomally integrated copy of the silent reporters. These clones exhibited intense blue colour with X-Gal staining solution.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
ADN
/
Proteínas Fúngicas
/
Secuencia de Bases
/
Línea Celular
/
Cricetinae
/
ADN Nucleotidiltransferasas
/
Animales
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
2003
Tipo del documento:
Artículo
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