Your browser doesn't support javascript.
loading
Binding characteristics and distribution of lactoferrin receptors in the gut and choroid plexus in newborn calves.
Indian J Exp Biol ; 2006 Oct; 44(10): 783-90
Artículo en Inglés | IMSEAR | ID: sea-63452
ABSTRACT
Lactoferrin (Lf), an iron-binding multifunctional glycoprotein, abundantly present in colostrum and milk of different species such as humans, bovines, and mice has been shown that bovine colostral Lf is transported into the CSF via plasma in newborn calves. Specific Lf-receptors (Lf-R) are present in different cells of different species. In the present study, we report for the first time, the presence and distribution of Lf-R in the intestine and choroid plexus in newborn calves. Brush-border membrane vesicles (BBMV) were prepared from the mucosa of duodenum, jejunum, ileum, colon, epithelium overlying Peyer's patches (EOPP) in jejunum (EOPPJ) and ileum (EOPPI), and choroid plexus membranes. Receptor binding assays were carried out using 125I labeled bovine Lf. Specific and saturable Lf-R were found in BBMV of all the intestinal segments and choroid plexus examined. Nonlinear regression and Scatchard plot analyses clearly revealed that EOPP had the highest binding maximal (Bmax), and lowest in colon. The maximum dissociation constant (Kd) 0.7 microM was in colon while, Bmax and Kd in choroid plexus membrane were 16.87 nmol/mg protein and 0.34 microM, respectively. All these findings together strongly suggested that Lf was transported into CSF via plasma through receptor mediated transcytosis.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Unión Proteica / Bovinos / Plexo Coroideo / Receptores de Superficie Celular / Sistema Digestivo / Animales / Animales Recién Nacidos / Microvellosidades Idioma: Inglés Revista: Indian J Exp Biol Año: 2006 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Unión Proteica / Bovinos / Plexo Coroideo / Receptores de Superficie Celular / Sistema Digestivo / Animales / Animales Recién Nacidos / Microvellosidades Idioma: Inglés Revista: Indian J Exp Biol Año: 2006 Tipo del documento: Artículo