Hepatitis G virus infection in India: prevalence and phylogenetic analysis based on 5' non-coding region.
Artículo
en Inglés
| IMSEAR
| ID: sea-64096
ABSTRACT
OBJECTIVES:
To determine the prevalence of hepatitis G virus (HGV) infection in western India and to carry out phylogenetic analysis of HGV isolates.METHODS:
Reverse transcription-polymerase chain reaction (RT-PCR) assay was used to detect HGV RNA in serum samples obtained from paid plasma donors, patients with hemophilia and voluntary blood donors. Nine Indian and one Kenyan HGV RNA-positive samples were sequenced in the 5' non-coding region (5'-NCR). Phylogenetic analysis based on the comparison of a 101 nucleotide fragment from a large number of HGV isolates from 22 countries (including Indian and Kenyan sequences obtained during the present study) was carried out.RESULTS:
HGV RNA positivity rates among paid plasma donors from a commercial plasmapheresis unit (7/43, 16.3%) and patients with hemophilia (5/44, 11.4%) were significantly higher than that in voluntary blood donors (0/51; p=0.003 and 0.019, respectively). Among patients with acute non-A to E hepatitis and fulminant hepatic failure, 1 of 50 and 1 of 28 were HGV RNA-positive, whereas 6 of 49 (12%) patients with chronic liver disease had circulating HGV RNA. All Indian isolates belonged to genotype 2, whereas the Kenyan isolate formed a distinct branch within genotype 1 consisting of African isolates.CONCLUSION:
Our results suggest existence of parenteral transmission of HGV in the Indian population. HGV was not an important cause of acute non-A to E hepatitis or fulminant hepatic failure among the patients investigated. Genotype 2 seems to be the most prevalent genotype in western India.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Filogenia
/
Femenino
/
Humanos
/
Masculino
/
ARN Viral
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Estudios Seroepidemiológicos
/
Prevalencia
/
Factores de Riesgo
Tipo de estudio:
Estudio de etiología
/
Estudio de prevalencia
/
Factores de riesgo
País/Región como asunto:
Asia
Idioma:
Inglés
Año:
2001
Tipo del documento:
Artículo
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