Effect of attachment surface on the growth and differentiation of breast cells.
Indian J Pathol Microbiol
;
1995 Oct; 38(4): 403-6
Artículo
en Inglés
| IMSEAR
| ID: sea-72965
ABSTRACT
This experimental work was undertaken to study the difference in cell growth due to different attachment surface. Three types of attachment surface were studied (i) collagenized surface (ii) glass surface and (iii) plastic surface. Rat tail collagen suspension was prepared and coated on culture flasks. Human breast epithelial cells and breast carcinoma cells were cultured for three weeks. Cell counts were made before and after one, two and three weeks of culture. Out of five cases only two survived for more than a week. The best survival was observed on plastic surface. Collagenized and glass surfaces had similar results. Thus plastic surface is probably the best compared to glass and collagenized surface, possibly because the plastic surface provides better adhesion to malignant cells. It was also seen that in plastic surface the cells get lined up around a basement membrane like structure growing a resemblance to ductal structure. This is contradictory to the prevalent view that collagenized surface is best for in vitro growth and differentiation.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Ratas
/
Propiedades de Superficie
/
Mama
/
Neoplasias de la Mama
/
Femenino
/
Humanos
/
Adhesión Celular
/
Diferenciación Celular
/
División Celular
/
Técnicas de Cultivo de Célula
Idioma:
Inglés
Revista:
Indian J Pathol Microbiol
Año:
1995
Tipo del documento:
Artículo
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