Detection of extended spectrum beta-lactamase from clinical isolates in Davangere.
Indian J Pathol Microbiol
;
2008 Oct-Dec; 51(4): 497-9
Artículo
en Inglés
| IMSEAR
| ID: sea-73356
ABSTRACT
Extended spectrum beta-lactamase enzymes (ESBLs) are enzymes that have the ability to hydrolyze oxyiminocephalosporins and infections by isolates producing them are often difficult to treat. A study to detect the presence of these enzymes in isolates was conducted by our hospital. A total of 207 non repetitive isolates were screened for resistance to any of five screening agents. Those with suspicious profiles were checked for ESBL production by double-disk approximation or a synergy test. The isolates were also subjected to a phenotypic confirmation test as recommended by CLSI (formerly NCCLS). Various cephalosporins-beta-lactamase inhibitor combinations were also tested. Of the 204 (98.5%) screen-positive isolates, only 126 (61.7%) were identified as ESBL producers. Of these, 26.1% of the isolates were positive by using the double-disk synergy test (DDST) method alone, 13.4% were positive using the method recommended by CLSI, and 60.3% of the isolates were positive by both the DDST and CLSI methods. We also report a high percentage of resistance to cefoxitin (96.8%) indicating changes in porins.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Beta-Lactamasas
/
Femenino
/
Humanos
/
Masculino
/
Pruebas de Sensibilidad Microbiana
/
Cefalosporinas
/
Resistencia a las Cefalosporinas
/
Resistencia betalactámica
/
Beta-Lactamas
/
Enterobacteriaceae
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Indian J Pathol Microbiol
Año:
2008
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS