Autofluorescence: a screening test for mycotic infection in tissues.
Indian J Pathol Microbiol
;
2008 Apr-Jun; 51(2): 215-7
Artículo
en Inglés
| IMSEAR
| ID: sea-74030
ABSTRACT
Fungal infection is a major health concern as the clinical features are not very distinctive. Lack of rapid diagnostic techniques results in delay in diagnosis, which may even culminate in a fatal outcome. The fact that many pathogenic fungal organisms autofluoresce in hematoxylin and eosin (H and E)-stained sections under ultraviolet illumination led us to evaluate the role of autofluorescence as a rapid screening technique for fungal infections. The aim of the present study was to assess the value of autofluorescence as a screening method for detecting fungi on tissue sections and to compare the results of autofluorescence with conventional histochemical stains for fungi. Hematoxylin and eosin-stained slides of mycotic lesions were examined under fluorescent microscope and the findings were compared with results of Gomori's methenamine silver and periodic acid-Schiff stains. We found fungal autofluorescence in 63 out of 64 cases studied, with a sensitivity of 97.8% and specificity of 100% in comparison with fungal stains. This was statistically significant (P < 0.05). We conclude that autofluorescence can be used as a rapid screening method for identification of fungi in tissue sections as it does not require any other specialized staining procedure.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Aspergilosis
/
Coloración y Etiquetado
/
Humanos
/
Candidiasis
/
Estudios Retrospectivos
/
Sensibilidad y Especificidad
/
Cigomicosis
/
Enfermedades Renales
/
Microscopía Fluorescente
/
Micología
Tipo de estudio:
Estudio diagnóstico
/
Estudios de evaluación
/
Estudio observacional
/
Estudio pronóstico
/
Estudio de tamizaje
Idioma:
Inglés
Revista:
Indian J Pathol Microbiol
Año:
2008
Tipo del documento:
Artículo
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