Polymerase chain reaction using IS6110 primer to detect Mycobacterium tuberculosis in clinical samples.
Indian J Pathol Microbiol
;
2000 Oct; 43(4): 395-402
Artículo
en Inglés
| IMSEAR
| ID: sea-74354
ABSTRACT
Nucleic acid amplification using IS6110 primers to detect Mycobacterium tuberculosis in clinical specimens has been extensively used as laboratory tool for the diagnosis for tuberculosis. Despite it's dramatic scientific value in practice, it is not as sensitive as expected for the detection of Mycobacterium tuberculosis. The results of the study suggest that PCR using 123 bp fragment of DNA belonging to IS6110 is specific (95.6%) but only has a sensitivity of 30% to detect M. tuberculosis in clinical specimens.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Tuberculosis
/
Humanos
/
ADN Bacteriano
/
Elementos Transponibles de ADN
/
Reacción en Cadena de la Polimerasa
/
Sensibilidad y Especificidad
/
Mycobacterium tuberculosis
Tipo de estudio:
Estudio diagnóstico
/
Estudios de evaluación
Idioma:
Inglés
Revista:
Indian J Pathol Microbiol
Año:
2000
Tipo del documento:
Artículo
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