Comparing serology, antigenemia assay and polymerase chain reaction for the diagnosis of cytomegalovirus infection in renal transplant patients.
Artículo
en Inglés
| IMSEAR
| ID: sea-86108
ABSTRACT
BACKGROUND:
Cytomegalovirus (CMV) disease is responsible for significant morbidity and mortality following renal transplantation. Currently serology is the only method widely available in our country. Newer methods like early CMV pp65 antigenemia assay and CMV DNA amplification can diagnose CMV disease in its very early period.AIM:
The aim of our study was to compare serologic method with antigenemia assay and CMV DNA amplification to diagnose CMV.METHODS:
Seventy-three renal transplant recipients (from 7 centres) with clinical suspicion of CMV disease were studied prospectively. The diagnosis of CMV infection was suspected on the basis of fever and leucopenia. RESULT ANDDISCUSSION:
Three tests were done in all 73 patients and in 22 healthy subjects (control group). The sensitivity and specificity of serological test (CMV IgM) was 72.97 and 62.06%; of antigenemia assay was 89.18 and 100% and of PCR was 100 and 72.41%.CONCLUSION:
Antigenemia assay is a sensitive and specific test for early and rapid diagnosis of CMV infection. Qualitative PCR is a sensitive marker but has low specificity.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Pronóstico
/
Femenino
/
Humanos
/
Masculino
/
Pruebas Serológicas
/
Estudios de Casos y Controles
/
Reacción en Cadena de la Polimerasa
/
Valor Predictivo de las Pruebas
/
Sensibilidad y Especificidad
/
Adolescente
Tipo de estudio:
Estudio diagnóstico
/
Estudio observacional
/
Estudio pronóstico
/
Investigación cualitativa
/
Factores de riesgo
Idioma:
Inglés
Año:
2004
Tipo del documento:
Artículo
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