Absorption and elution test and real time PCR in ABO blood group identification: A comparative study / 中国输血杂志

ABSTRACT

【Objective】 To evaluate the performance of absorption and elution test and real time PCR in identifying ABO blood group. 【Methods】 The blood samples with weakened antibody expression serologically were screened and analyzed by absorption and elution test, real time PCR and gene sequencing. The gene sequencing results were used as the gold standard to assess the consistency of the other two methods, and the sensitivity and specificity were compared. 【Results】 Compared with the sequencing results (gold standard), absorption and elution test showed moderate consistency, while real time PCR showed good consistency. Both the sensitivity and specificity of absorption and elution test and real time PCR were statistically different. 【Conclusion】 There was a good agreement between real time PCR and gene sequencing in identifying suspicious ABO blood group with weakened antibody expression serologically. Real time PCR, with a low misjudgment rate, has strong ability to detect weak antigen and is more conducive to identify blood type compared with absorption and elution test.