HPLC specific chromatogram of Vernonia anthelmintica and determination of six components / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 910-915, 2020.
Artículo
en Chino
| WPRIM
| ID: wpr-1008518
ABSTRACT
This work aims to establish an HPLC specific chromatogram and determine six components of Vernonia anthelmintica with chlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, scutellarein and luteolin as index components. HPLC analysis was performed on a Waters Xbridge C_(18) column(4.6 mm×250 mm, 5 μm) with gradient elution of acetonitrile-0.05% trifluoroacetic acid solution at a flow rate of 1.0 mL·min~(-1). The detection wave length was 360 nm and the column temperature was 30 ℃. Chemometrics software Chempattern was employed to analyze the data. HPLC specific chromatogram of V. anthelmintica was established and six characteristic peaks were marked. Six characteristic peaks were simultaneously determined by HPLC within 50 min. The contents of the six components in 13 batch samples of V. anthelmintica were 0.14%-0.68%, 0.44%-0.74%, 0.63%-1.01%, 0.14%-0.71%, 0.15%-0.26% and 0.010%-0.030%, respectively. The HPLC specific chromatogram of V. anthelmintica, together with determination of six components showed strong specificity, and it can be used for the quality control of the crude drug.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Control de Calidad
/
Medicamentos Herbarios Chinos
/
Cromatografía Líquida de Alta Presión
/
Vernonia
/
Fitoquímicos
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2020
Tipo del documento:
Artículo
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