Combination of stromal vascular fraction and Ad-COMP-Ang1 gene therapy improves long-term therapeutic efficacy for diabetes-induced erectile dysfunction / 亚洲男科学杂志(英文版)
Asian j. androl
; Asian j. androl;(6): 465-472, 2018.
Article
en En
| WPRIM
| ID: wpr-1009603
Biblioteca responsable:
WPRO
ABSTRACT
Men with diabetic erectile dysfunction (ED) respond poorly to the currently available oral phosphodiesterase-5 inhibitors. Therefore, functional therapies for diabetic ED are needed. Stromal vascular fraction (SVF) and the adenovirus-mediated cartilage oligomeric matrix angiopoietin-1 (Ad-COMP-Ang1) gene are known to play critical roles in penile erection. We previously reported that SVF and Ad-COMP-Ang1 have only a short-term effect in restoring erectile function. Further improvements to ED therapy are needed for long-lasting effects. In the present study, we aimed to test if the combination of SVF and Ad-COMP-Ang1 could extend the erection effect in diabetic ED. We found that the combination therapy showed a long-term effect in restoring erectile function through enhanced penile endothelial and neural cell regeneration. Combination therapy with SVF and Ad-COMP-Ang1 notably restored cavernous endothelial cell numbers, pericyte numbers, endothelial cell-cell junctions, decreased cavernous endothelial cell permeability, and promoted neural regeneration for at least 4 weeks in diabetic mice. In summary, this is an initial description of the long-term effect of combination therapy with SVF and Ad-COMP-Ang1 in restoring erectile function through a dual effect on endothelial and neural cell regeneration. Such combination therapy may have therapeutic potential for the treatment of diabetic ED.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Permeabilidad
/
Erección Peniana
/
Endotelio Vascular
/
Terapia Genética
/
Angiopoyetina 1
/
Trasplante de Células Madre Mesenquimatosas
/
Diabetes Mellitus Experimental
/
Disfunción Eréctil
/
Uniones Intercelulares
Límite:
Animals
Idioma:
En
Revista:
Asian j. androl
Año:
2018
Tipo del documento:
Article