Cytogenetic test and clinical study on cryptic acute promyelocytic leukemia with ins (15; 17) / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 843-847, 2019.
Artículo
en Chino
| WPRIM
| ID: wpr-1012078
ABSTRACT
Objective:
To investigate the genetic screening methods for cryptic acute promyelocytic leukemia (APL) to further explore its clinical prognosis.Methods:
From June 2016 to November 2018, we collected 373 newly diagnosed APL cases. The patients were retrospected by the results of PML-RARα detections both by RT-PCR and i-FISH, those who harbored positive PML-RARα detection by RT-PCR and negative by i-FISH were chosen. Metaphase FISH and Sanger sequencing were further performed to verify these results.Results:
A total of 7 cryptic APL cases were discovered. These cases had tiny fragment of RARα inserted into PML in chromosome 15, formed ins (15;17) . The 7 cryptic APL cases had no PML-RARα gene subtype specificity, involving 5 cases in L subtype, 1 case in S subtype and 1 case in V subtype respectively. After the treatment of retinoic acid and arsenic or anthracyclines, 6 cases achieved complete remission, 1 case died of intracranial hemorrhage on the 6th day of therapy.Conclusion:
The size and covering position of PML-RARα probe should be taken into account when PML-RARα was performed by FISH on APL patients. Furthermore, combination with Metaphase FISH could improve the recognition of cryptic APL. There were no differences between the cryptic and common APL patients in terms of clinical features and treatment choices. Cryptic APL patients also had a good response to the therapy of retinoic acid and arsenic or anthracyclines.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Tretinoina
/
Cromosomas Humanos Par 15
/
Cromosomas Humanos Par 17
/
Leucemia Promielocítica Aguda
/
Proteínas de Fusión Oncogénica
/
Hibridación Fluorescente in Situ
/
Citogenética
/
Receptor alfa de Ácido Retinoico
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Hematology
Año:
2019
Tipo del documento:
Artículo
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