The protective effect of MANF on intestinal epithelial cell injury induced by endoplasmic reticulum stress / 中国药理学通报

ABSTRACT

Aim To observe the proteetive effect of meseneephalie astrocyte-de rived neurotrophic faetor (MANF) on intestinal epithelial eells under endoplas- mic reticulum stress (EH stress).Methods Normal human intestinal epithelial cell line FHs74Int was stimulated with EH stress inducers, tunicmycin (TM) and TNF-cx, then the expression of endogenous MANF was observed.The recombinant plasmids MANF-GFP and GFP were transfected into FHs74Int cells individually, the transfection efficiency was observed by fluorescence j j microscopy, and the effect of MANF on EH stress was observed by Western blot.The effect of MANF on the proliferation of intestinal epithelial cells stimulated by TM was detected via CCK-8 assay.The effect of MANF on apoptosis after EH stress was detected by Western blot and flow cytometry.Results EH stress could induce the expression of endogenous MANE in intestinal epithelial cells.Overexpression of MANE significantly inhibited the expression of the EH stress-related proteins, Bip and CHOP, and promoted the proliferation of intestinal epithelial cells.At the same time, it could reduce the production of the proapoptotic proteins cleaved-c a spa se-3 and Bax, increase the expression of the antiapoptotic protein Bcl-2, and inhibit the proportion of early and late apoptosis of intestinal epithelial cells.Conclusions MANF plays a protective role in inhibiting EH stress in intestinal epithelial cells by promoting cell proliferation and reducing apoptosis.