Salvianolic acid B reduces liver inflammation in mice with atherosclerosis by inhibiting MAPKs/NF-kB signaling pathway / 中国药理学通报

ABSTRACT

Aim To explore the effeet of salvianolic aeirl B on liver inflammation in atheroselerosis model mice and its mechanism.Methods Thirty-two male LDLR mice were randomly divided into control group, model group, salvianolic acid B group, and ator- vastatin group.The control group was fed with ordinary feed, and the other groups were fed with high-fat feed for 12 weeks.The control group and the model group were injected intraperitoneally with normal saline, the salvianolic acid B group was injected intraperitoneally with the salvianolic acid B solution,and the atorvastatin group was given intragastrically with atorvastatin solution for 12 weeks.Biochemical detection methods were used to detect the serum TC, TG, AST and ALT values of mice.Oil red 0 staining was used to observe mouse aortic sinus plaque area, and HE staining was used to observe pathological changes of mouse liver.ELISA and RT-PCR methods were used to detect serum IL- 1 p, 1L-6,and TNF-ot levels,and 1L-1 (3, 1L-6,and TNF-a mRNA in liver.Western blot was used to determine the protein expression of VCAM, iNOS, JNK, p38, ERK1/2, IkB, and NF-kB in mouse liver tissues.Results Compared with model group, salvianolic acid B and atorvastatin reduced the levels of serum TC, TG, AST,and ALT in mice ( P <0.05 ) ,reduced the plaque area of aorta in mice, and improved the pathological changes of liver tissues,and down-regulated mouse serum IL-l (3, IL-6, TNF-cx content and mRNA levels (P < 0.05 ).Salvianolic acid B reduced the protein levels of VCAM and iNOS in liver tissues of mice,as well as the phosphorylation levels of JNK, p38 , ERK1/2 , IkB , and NF-kB proteins ( P <0.05 ).Conclusions Salvianolic acid B reduces liver inflammation in atherosclerotic model mice,which may be related to its inhibition of MAPKs/NF-kB signaling pathway.