Effect of microRNA-125b on proliferation, apoptosis and collagen expression of hepatic stellate cells / 中国药理学通报

ABSTRACT

Aim To investigate the effect of miR-125b on HSC proliferation, apoptosis and collagen expression. Methods HSCs were transfected with miR- 125b mimics and inhibitors, and then molecular biology methods were used to detect the expression of Collagen I and HI in HSCs; MIT method was employed to detect HSC proliferation, and flow cytometry to detect HSC apoptotic rate. Results Molecular biological testing found that miR-125b mimics inhibited the expression of Collagen I and HI, and miR-125b inhibitors promoted the expression of Collagen I and HI mRNA; miR-125b mimics inhibited the proliferation of HSC, and miR-125b inhibitors promoted HSC proliferation; miR-125b mimics promoted HSC cell apoptosis, miR- 125b inhibitor inhibited HSC apoptosis, the above experimental results were statistically significant compared with its negative control group ( P < 0. 05 ). Conclusions miR-125b has anti-liver fibrosis effects, which may be related to the regulation of the phenotype of hepatic stellate cells.