A comparative study on rat models of oral mucosal ulcers caused by several anti-cancer chemotherapy drugs / 中国药理学通报

ABSTRACT

;Aim To compare the characteristics of rat oral ulcer models caused by different chemotherapeutic drugs in order to find a stable oral ulcer model caused by chemotherapy drugs. Methods Except the control group, each group was injected with corresponding concentrations of chemotherapeutic drugs in the right buccal mucosa. During the experiment, the weight, food consumption, oral ulcer diameter were recorded; at the end of the experiment, ulcer diameter, oral mucosa histopathology, oral mucosa local cytokine level, organ index and T lymphocytes subtype in peripheral blood were observed. Results The body weight and food consumption of the animals in each group were significantly lower than those in control group after injection of chemotherapy drugs; obvious oral mucosal ulceration was observed in DDP group and PTX group, and broken and shed mucosa were observed by optical microscope in the two groups. The level of IL-lß mRNA in local mucosal tissues significantly increased, and the level of EGFR mRNA was significantly reduced in the tow groups, but organ index did not change significantly. Ulcers were not observed in MTX, VLB, and AMD groups, but organ index and cytokine significantly decreased. T lymphocyte subtypes had no difference among the emimals in each group. Conclusions Injecting cisplatin or paclitaxel in rat oral mucosa can induce a stable oral ulcer model, which can be used in therapeutic evaluation of anti-oral ulcer drugs by observing ulcer diameter, food consumption, cytokines level etc.