Effect of exosomes derived from bone marrow mesenchymal stem cells on the polarization of mouse liver Kupffer cells / 解剖学报

ABSTRACT

Objective To investigate the the effect of exosomes derived from bone marrow mesenchymal stem cells (BMSCs) on the polarization of liver Kupffer cells. Methods After BMSCs were isolated and cultured in vitro, their surface molecular expression was identified by flow cytometry, and their differentiation potential was induced and identified by osteogenic and lipogenic induction media. Exosomes were extracted from the supernatant of BMSCs culture by exosomes extraction kit, and their morphology was observed by electron microscopy, and surface molecular expression was identified by flow cytometry. In vitro cultured Kupffer cells were randomly divided into normal culture, lipopolysaccharide (LPS) stimulation and LPS co-culture groups, and morphological changes in Kupffer cells were observed under light microscope. Sixty mice were injected intraperitoneally with CC1