Effects of resveratrol on microglia polarization after oxygen glucose deprivation / reoxygenation injury in vitro / 解剖学报

ABSTRACT

Objective To investigate the effect of resveratrol on microglia polarization after oxygen glucose deprivation / reoxygenation (OGD / R) in vitro. Methods N9 microglias were cultured under oxygen-glucose deprivation for 150 minutes and reoxygenation for 24 hours. The experiment was divided into normal, control and resveratrol pretreatment groups. Cell viability was measured by cell counting kit-8(CCK-8) assay. Malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity in the supernatant were detected by thiobarbituric acid(TBA) and water soluble terazolium salt (WST-1) respectively. Nuclear factor E2-related factor 2 (Nrf2) nuclear translocation was detected by immunofluorescence. The levels of CD206, inducible nitric oxide synthase (iNOS), Nrf2, heme oxygenase-1 (HO-1), NAD(P) H quinone oxidoreductase 1(NQO1) protein were measured by Western blotting. The mRNA levels of CD206, iNOS were detected by Real-time PCR. Results After OGD / R injury, cell viability, SOD activity, CD206, Nrf2, HO-1, NQO1 protein or mRNA expression in resveratrol groups were significantly higher than those in the control group (P<0. 05, n = 3), while MDA content and iNOS protein and mRNA expression were significantly lower than those in the control group (P<0. 05, n = 3) . Nrf2 protein in resveratrol group was significantly transferred to the nucleus compared with the control group. Conclusion Resveratrol pretreatment may regulate M1 / M2 polarization of microglia and reduce oxidative stress after OGD / R via strengthening activation of Nrf2.